Part:BBa_K4633104

Bacillus subtilis L-arabinose induced system

This composite construct features two distinct systems, the first is meant to allow for the expression of the araR protein, a repressor from Bacillus subtilis 168 that only allows for transcription in the presence of L-arabinose. The first system is composed of the native promoter – paraR (BBa_K4633004), followed by the native ribosomal binding site (RBS) (BBa_K4633005) and the protein araR (BBa_K4633003). Downstream this system is the T1 terminator (BBa_B0010) to ensure that the two systems won’t interfere with one another.

The second system is the inducible system, it is composed of paraE (BBa_K4633006), a promoter containing araR binding sites and thus can be inhibited by araR, its native RBS (BBa_K4633007) and lastly mCherry (BBa_J06504), to allow for characterization of the system.

Usage and Biology

This composite part, along with two other composite parts BBa_K4633102 and BBa_K4633103, was meant to test the basic parts designed by the 2023 Technion team. We were not able to test this system and we hope to do so after the wiki freeze.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 186
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 213